HP Data Explorer 4 Series Manual do Utilizador descarregar pdf (Página 257)

100

101

Adjusting the Baseline

Data Explorer

™

Software User’s Guide 5-53

NOTE: Flexibility and Degree parameters may require

some testing to determine the optimum settings for your

data.

4. Click OK.

The baseline is adjusted, and the trace is displayed with a

AdvBC trace label.

Degree Determines how closely the calculated baseline fits the

data. Valid entries are 0.0 to 1.0.

A value closer to 1 fits the baseline more closely to the

data, and corrects the midpoint of the noise signal to

approximately 0 intensity.

A value closer to 0 fits the baseline less closely to the

data, and corrects the midpoint of the noise signal to a

value greater than 0 intensity (see below).

For protein and tryptic digest peptide spectra, use 0 to

0.5. If the baseline after correction is too high, increase

the Degree value (see below).

A lower Degree setting allows the baseline correction to

occur more quickly, and often provides better correction

than a higher setting.

Parameter Description/Specifies

Degree=1

Midpoint of noise signal

corrected to 0

Degree=0

Midpoint of noise signal

corrected above 0

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