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Chapter 7 Data Explorer Examples

7-4 Applied Biosystems

7.1.2 Deconvoluting and

Evaluating Unresolved

Chromatographic Peaks

Overview You can use the Data Explorer software to deconvolute

chromatographic peaks and obtain masses for each

component by:

• Creating a combined spectrum to identify the coeluting

species by mass

• Creating extracted ion chromatograms for the masses,

and comparing the extracted ion chromatograms to the

original TIC containing the unresolved peaks

You can then create a combined spectrum from each

extracted ion chromatogram to evaluate the spectral data.

Creating a

combined

spectrum

The following example illustrates how to deconvolute two

unresolved chromatographic peaks in a tryptic digest of

cytochrome c:

1. Display the data file.

2. In the Chromatogram window, zoom in on the unresolved

peaks (Figure 7-2).

Figure 7-2 Unresolved Peaks in Cytochrome C

3. In the Chromatogram window, right-click-drag over the

unresolved peak pair.

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